ASI & Imaging X symposium @ Labtechnology 2016

During the Labtechnology 2016 conference on June 15 – 16, ASI will be present as an exhibitor at booth #D19. Here we will show you how you can benefit from our technology in Life science application such as mass spectrometry, electron microscopy and color X-ray CT.

Next to the exhibition ASI organises the Imaging X symposium on the 15th of June. “Imaging X” tries to indicate that this session includes different types of imaging techniques, i.e. the X stands for a free variable. The conference is aiming at an audience that is involved in Life Sciences with a focus on pharmaceuticals. Here the benefits of different techniques are highlighted with regards to discovery based imaging and how this could support understanding deceases and how pharmaceuticals can be tracked & traced.

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Program:
13:00 – 14:00 | Dr. Ellis Shane from M4I Maastricht University
Title: Imaging for the Masses: Multiplexed molecular Imaging Using Mass Spectrometry

Description:
Mass spectrometry imaging (MSI) provides a powerful approach for studying the localized chemical processes occurring within tissues and cells. Unlike other imaging techniques MSI facilitates the simultaneous imaging of thousands of molecules in a single experiment without the need for external labels and with excellent molecular specificity. In recent years MSI has emerged as a highly promising technique for understanding and diagnosing diseased tissues based on their localized molecular profiles- an approach often referred to as “molecular histology”.  After an introduction to the concept of MSI this lecture will focus on recent technological advancements enabling higher throughout and sensitivity and the new capabilities these are now offering for molecular imaging research and molecular diagnostics. For example, we will show how MSI offers exciting possibilities for evaluating tissue viability for transplants, revealing molecular heterogeneity in tumors, even for 3D imaging and even intra operative diagnostics.

Secondly, we will discuss recent work from our group using new ion detectors initially developed for high energy physics (Medipix/Timepix family). These detectors provide dramatically improved sensitivity and parallelized imaging functionality. The use of highly sensitive active pixel detectors allows up to two orders of magnitude improvement in the detection of large, intact molecules such as antibodies compared to conventional detectors under identical conditions while also allowing fundamental ion optical processes to be directly studies and exploited. We will show how these combined features allow the stigmatic imaging of biomolecules from surfaces whereby many pixels are acquired in parallel with a resolution independent of the laser spot size, thus providing an alternative route towards high throughout imaging.

14:00 – 15:00 | Dr. Raimond Ravelli from Leiden University Medical Center (LUMC)
Title: The resolution revolution in cryo-electron microscopy and its impact on structural biology

Description:
Structural biologists study the three-dimensional (3D) structure of large biomolecules such as proteins and RNA in order to gain a better understanding of their function. The most commonly used method to achieve high resolution structures is X-ray diffraction, for which crystals will need to be obtained from the biomolecule of interest. Nuclear magnetic resonance (NMR) offers the advantage that, with restraints on protein size and labelling, both structural and dynamic information can be acquired.

Currently there is a revolution going on in the application of cryo-electron microscopy for structural biology. Only three years ago, researchers were able to build the first de novo 3D structures of asymmetric macromolecular complexes from data obtained with a cryo-electron microscope. These large complexes did not have to be crystallized, but are, molecule by molecule, imaged at cryogenic temperatures within an electron microscope. Tens to hundreds of thousands of individual images of molecules in random orientations are subsequently joined together computationally to form a three-dimensional map into which the model can be built.

Over the past three years, numerous applications of this technique have been reported in literature. The limits of what seems to be possible in terms of resolution and complex size, have been pushed multiple times. In my lecture I will share these developments with you, discuss its relevance to biology and human health, and give a vision of how cryo-electron microscopy could further develop within the coming years to come.

Location:
Jaarbeurs Utrecht, The Netherlands

More info TBA

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