TOF-Mass Spectrometry Imaging
MSI in microscope mode
In MSI, there are two ways to perform mass spectrometry imaging，1. microprobe mode; 2. microscope mode.
Microprobe
The laser was focused onto the sample surface to ionize the sample. The spatial resolution is restricted by the spot-size of the laser. Each scanning point as a “pixel” in latter image has a mass spectrum.
Microscope
By contrast, the spot-size of the laser is normally bigger (100 – 300 um) than the one (1 – 10 um) used in the microprobe mode, while requires time- and position- sensitive detector to image the focused area. By doing this, it can speed up the data acquisition, and increase spatial resolution.
Advantages of using Microscope mode
- High speed
- Large image area
- High spatial resolution
- High mass sensitive
15000x faster than rapifleX according to M4I work.
[two images (from internet) shown left HAVE TO BE REDREW due to copyright issue!! CCD camera should be replaced by the TPX3CAM]
[Some nice experimental results/images may be asked and obtained from M4I]
[For commercial use, always ask for permission first before any use!]
Relevant imaging techniques
Microscope mode mass spectrometry imaging can be applied for:
- MALDI-TOF
- SIMS-TOF
[It could be good to show some example results from M4I, e.g. sample images, publications, etc.
Experimental setup which integrates the TPX3CAM]